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myr ha akt1  (Addgene inc)


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    Structured Review

    Addgene inc myr ha akt1
    Myr Ha Akt1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 80 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcdna3+myr+ha+akt1/1036+pcDNA3+Myr+HA+Akt1+(Plasmid+%239008)/pmc13010091-71-0-6
    Average 93 stars, based on 80 article reviews
    myr ha akt1 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Inhibition of Janus Kinase 1 synergizes docetaxel sensitivity in prostate cancer cells
    Article Snippet: .. Ubc.GFP was a gift from Linzhao Cheng (Addgene plasmid # 24983; http://n2t.net/addgene:24983 ; RRID:Addgene_24983), and pcDNA3 Myr HA Akt1 was a gift from William Sellers (Addgene plasmid # 9008; http://n2t.net/addgene:9008 ; RRID:Addgene_9008). .. Plasmids were transfected into target cells using X‐tremeGENE HP DNA transfection reagent (Roche Diagnostic, Indianapolis, IN, USA), according to the manufacturer's protocol.

    Article Title: Selective Inhibitors of mTORC1 Activate 4EBP1 and Suppress Tumor Growth
    Article Snippet: A homogeneous assay mixture was prepared using 150 nM recombinant human FKBP12 fused to Emerald Green Fluorescent Protein (EmGFP), 2 nM GST-tagged human mTOR (residues 1360–2549), and 1 nM LanthaScreen Tb-anti-GST antibody (Rabbit, Thermo Fisher Scientific Cat # A15112) in 50 mM HEPES pH 7.5, 0.01% Polysorbate 20, 1 mM EGTA, and 10 mM MnCl 2 . .. Gene overexpression cell lines MCF-7 cells overexpressing a myristoylated form of AKT (Myr-AKT) were generated by transfection with 1 µg of pcDNA3-Myr-HA-AKT1 (Addgene plasmid #9008). ..

    Article Title: RBL1/p107 Expression Levels Are Modulated by Multiple Signaling Pathways
    Article Snippet: HEK-293FT supernatants were used to transduce A549 and MSTO-211H cells in the presence of 8 μg/mL polybrene (Sigma-Aldrich). .. pcDNA3 Myr HA Akt1 (Addgene plasmid #9008) and pcDNA3 Myr HA Akt2 (Addgene plasmid #9016) were a gift from William Sellers [ ]. .. The following plasmids were used to obtain the cDNAs encoding CaMKIα, CaMKIIα and CaMKIV: pDONR223-CAMK1 (Addgene plasmid # 23544), pDONR223-CAMK2A (Addgene plasmid # 23408) and pDONR223-CAMK4 (Addgene plasmid # 23812).

    other:

    Article Title: Selective inhibitors of mTORC1 activate 4EBP1 and suppress tumor growth
    Article Snippet: 1Department of Biology, Revolution Medicines, Inc., Redwood City, CA, USA.. 2Louis V. Gerstner Jr. Graduate School of Biomedical Sciences, Memorial Sloan-Kettering Cancer Center, New York, NY, USA.. 3Program in Molecular Pharmacology, Department of Medicine, Memorial Sloan-Kettering Cancer Center (MSKCC), New York, NY, USA.

    Article Title: Selective Inhibitors of mTORC1 Activate 4EBP1 and Suppress Tumor Growth
    Article Snippet: Kinase selectivity and off-target profile were determined using the KiNativTM platform and performed at ActivX Biosciences.

    Over Expression:

    Article Title: Selective Inhibitors of mTORC1 Activate 4EBP1 and Suppress Tumor Growth
    Article Snippet: A homogeneous assay mixture was prepared using 150 nM recombinant human FKBP12 fused to Emerald Green Fluorescent Protein (EmGFP), 2 nM GST-tagged human mTOR (residues 1360–2549), and 1 nM LanthaScreen Tb-anti-GST antibody (Rabbit, Thermo Fisher Scientific Cat # A15112) in 50 mM HEPES pH 7.5, 0.01% Polysorbate 20, 1 mM EGTA, and 10 mM MnCl 2 . .. Gene overexpression cell lines MCF-7 cells overexpressing a myristoylated form of AKT (Myr-AKT) were generated by transfection with 1 µg of pcDNA3-Myr-HA-AKT1 (Addgene plasmid #9008). ..

    Generated:

    Article Title: Selective Inhibitors of mTORC1 Activate 4EBP1 and Suppress Tumor Growth
    Article Snippet: A homogeneous assay mixture was prepared using 150 nM recombinant human FKBP12 fused to Emerald Green Fluorescent Protein (EmGFP), 2 nM GST-tagged human mTOR (residues 1360–2549), and 1 nM LanthaScreen Tb-anti-GST antibody (Rabbit, Thermo Fisher Scientific Cat # A15112) in 50 mM HEPES pH 7.5, 0.01% Polysorbate 20, 1 mM EGTA, and 10 mM MnCl 2 . .. Gene overexpression cell lines MCF-7 cells overexpressing a myristoylated form of AKT (Myr-AKT) were generated by transfection with 1 µg of pcDNA3-Myr-HA-AKT1 (Addgene plasmid #9008). ..

    Transfection:

    Article Title: Selective Inhibitors of mTORC1 Activate 4EBP1 and Suppress Tumor Growth
    Article Snippet: A homogeneous assay mixture was prepared using 150 nM recombinant human FKBP12 fused to Emerald Green Fluorescent Protein (EmGFP), 2 nM GST-tagged human mTOR (residues 1360–2549), and 1 nM LanthaScreen Tb-anti-GST antibody (Rabbit, Thermo Fisher Scientific Cat # A15112) in 50 mM HEPES pH 7.5, 0.01% Polysorbate 20, 1 mM EGTA, and 10 mM MnCl 2 . .. Gene overexpression cell lines MCF-7 cells overexpressing a myristoylated form of AKT (Myr-AKT) were generated by transfection with 1 µg of pcDNA3-Myr-HA-AKT1 (Addgene plasmid #9008). ..



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